Defining the Role of RBBP4 in Oocyte Maturation and Preimplantation Development Using Trim-Away

Retinoblastoma-binding protein 4 (RBBP4) is a subunit of chromatin remodeling factor 1 (CAF-1) and is essential for mammalian oocyte maturation, embryo survival, and embryo implantation. RBBP4 also localizes to the chromatin and is a ubiquitously expressed nuclear protein. Previous methods used to study this protein include short interfacing RNAs (siRNAs) and CRISPR/Cas9. These techniques have limitations such as determining an indirect depletion of proteins, may trigger compensatory mechanisms, and may not be useful in non-dividing primary cells. A new, acute, and rapid endogenous protein depletion technique called Trim-Away, can overcome these limitations. Trim-Away is also widely applicable since it can be used with many off-the-shelf reagents. Trim-Away utilizes the TRIM21-antibody interaction within the cytosol and the ubiquitin-proteasome pathway (UPP) to target and degrade a protein of interest. Studying RBBP4 using Trim-Away can offer insight into possible new functions of RBBP4 and its maternal effect, and increase the knowledge on a new, acute, and endogenous protein depletion technique. Here we report that, RBBP4 is required for proper blastocyst development and RBBP4 is more abundant in MII oocytes than GVBD oocytes. We also report that the loss of RBBP4 hinders RNA synthesis and causes cell death in later stages of embryo development. While our Trim-Away methodology can deplete RBBP4 as early as the 2-cell stage in embryos, our oocyte Trim-Away protocol needs to be optimized

Poisson noise channel with dark current: Numerical computation of the optimal input distribution

This paper considers a discrete time-Poisson noise channel which is used to model pulse-amplitude modulated optical communication with a direct-detection receiver. The goal of this paper is to obtain insights into the capacity and the structure of the capacity-achieving distribution for the channel under the amplitude constraint $\mathsf{A}$ and in the presence of dark current $\lambda$. Using recent theoretical progress on the structure of the capacity-achieving distribution, this paper develops a numerical algorithm, based on the gradient ascent and Blahut-Arimoto algorithms, for computing the capacity and the capacity-achieving distribution. The algorithm is used to perform extensive numerical simulations for various regimes of $\mathsf{A}$ and $\lambda$.Comment: Submitted to IEEE ICC 2022. This is a companion paper of: A. Dytso, L. Barletta and S. Shamai Shitz, "Properties of the Support of the Capacity-Achieving Distribution of the Amplitude-Constrained Poisson Noise Channel," in IEEE Transactions on Information Theory, vol. 67, no. 11, pp. 7050-7066, Nov. 202

Effect of a finite external heat transfer coefficient on the Darcy-Benard instability in a vertical porous cylinder

The onset of thermal convection in a vertical porous cylinder is studied by considering the heating from below and the cooling from above as caused by external forced convection processes. These processes are parametrised through a finite Biot number, and hence through third-kind, or Robin, temperature conditions imposed on the lower and upper boundaries of the cylinder. Both the horizontal plane boundaries and the cylindrical sidewall are assumed to be impermeable; the sidewall is modelled as a thermally insulated boundary. The linear stability analysis is carried out by studying separable normal modes, and the principle of exchange of stabilities is proved. It is shown that the Biot number does not affect the ordering of the instability modes that, when the radius-to-height aspect ratio increases, are displayed in sequence at the onset of convection. On the other hand, the Biot number plays a central role in determining the transition aspect ratios from one mode to its follower. In the limit of a vanishingly small Biot number, just the first (non-axisymmetric) mode is displayed at the onset of convection, for every value of the aspect ratio. (C) 2013 American Institute of Physic